Microlisa HIV Ag & Ab 4th Generation ELISA Test Kit
Pack Sizes Available
Product Description
Technical Specifications
- Test Principle: 4th Generation Sandwich ELISA (enzyme-linked immunosorbent assay)
- Detection Targets: HIV-1 antibodies (Groups M & O, all subtypes including subtype C), HIV-2 antibodies, HIV-1 p24 antigen
- Specimen Type: Human serum or plasma (EDTA, heparin, or citrate)
- Test Format: 96-well microplate with breakaway strips (12 strips x 8 wells)
- Coated Antigens/Antibodies: HIV envelope proteins: gp41, C-terminus of gp120 (HIV-1), gp36 (HIV-2); Anti-p24 monoclonal antibodies
- Conjugate: Horseradish peroxidase (HRPO) conjugated HIV antigens and anti-p24 antibodies
- Incubation Temperature: 37°C ± 2°C
- Total Assay Time: Approximately 120 minutes (60 min specimen incubation + 30 min conjugate + 30 min substrate)
- Detection Wavelength: 450 nm (with optional 620-650 nm reference filter)
- Sensitivity: 100% (tested with seroconversion panels); p24 antigen sensitivity: 200 pg/ml
- Specificity: >99.5% (tested against large sample populations)
- Storage Conditions: 2-8°C (refrigerated), do not freeze
- Shelf Life: 24 months from date of manufacture when stored properly
- Regulatory Status: CE marked, meets ISO 15223-1 standards for IVD medical devices
Frequently asked questions
4th generation HIV ELISA kits like Microlisa HIV Ag & Ab detect both HIV antibodies (HIV-1 and HIV-2) and HIV-1 p24 antigen simultaneously, while 3rd generation tests detect antibodies only. This dual detection shortens the diagnostic window period by approximately 1-2 weeks, allowing identification of acute HIV infection during the seroconversion phase when p24 antigen is present but antibodies have not yet developed to detectable levels. This is critical for blood donor screening and early diagnosis.
The Microlisa HIV Ag & Ab test is designed for use with human serum or plasma. Both specimen types are acceptable. Plasma can be collected using EDTA, heparin, or citrate as anticoagulants. Specimens should be tested fresh or stored at 2-8°C for short-term storage (up to 7 days) or frozen at -20°C or below for longer storage. Avoid repeated freeze-thaw cycles which can affect test performance.
The Microlisa HIV Ag & Ab ELISA kit is available in multiple pack sizes: 96 tests (standard), 48 tests, and 480 tests (bulk). The 96-test kit contains breakaway microwell strips (12 strips of 8 wells each), allowing flexible testing from single specimens to full plate runs. The kit includes all necessary reagents: coated microwells, controls, conjugates, substrate, wash buffer, and stop solution.
The total assay procedure time is approximately 120 minutes (2 hours) from specimen addition to final reading. This includes: 60 minutes incubation for specimen and controls, washing steps, 30 minutes incubation with conjugate, washing, 30 minutes substrate incubation, and final reading at 450nm. The assay requires strict adherence to incubation times and temperatures (37°C ± 2°C) for accurate results.
Yes, Microlisa HIV Ag & Ab is designed to detect all major HIV-1 subtypes including Group M (subtypes A-H), Group O (which some tests miss), and subtype C which is particularly prevalent in India and Sub-Saharan Africa. The test also detects HIV-2 antibodies. This broad reactivity profile makes it suitable for screening diverse populations where multiple HIV subtypes may circulate.
No, Microlisa HIV Ag & Ab is a screening test, not a confirmatory test. All initially reactive samples must be retested in duplicate. Repeatedly reactive samples should be confirmed using supplemental tests such as Western Blot, Immunoblot, or PCR (nucleic acid testing). A negative screening result does not absolutely rule out HIV infection during the very early window period or in severely immunosuppressed individuals. Clinical correlation and appropriate follow-up testing are essential.
Advantage PAN Malaria Card Rapid Diagnostic Test Kit
Malaria diagnosis in endemic regions operates under time pressure that microscopy cannot always accommodate. A patient presenting with fever in a rural health center at midnight does not have the luxury of waiting until morning for a trained microscopist to arrive, prepare slides, and spend twenty minutes examining blood films under oil immersion. That delay can mean the difference between timely artemisinin treatment and cerebral malaria developing overnight. Advantage PAN Malaria Card was designed to deliver species-level diagnosis in settings where microscopy is impractical or unavailable. This is a rapid visual immunoassay for qualitative detection of all four human Plasmodium species (P. falciparum, P. vivax, P. malariae, P. ovale) based on pan-specific plasmodium lactate dehydrogenase (pLDH) antigen in whole blood. The test provides results within 20 minutes using a simple fingerstick blood sample, no laboratory equipment required, making it ideal for point-of-care testing in primary health centers, rural clinics, field hospitals, and outbreak response settings. The assay is based on sandwich immunochromatography using monoclonal antibodies specific to pLDH, an enzyme produced by all Plasmodium species during their erythrocytic life cycle. When infected blood is added to the test device and assay buffer is applied, red blood cells lyse and pLDH antigen (if present) binds to gold-conjugated anti-pLDH antibodies. This complex migrates along the nitrocellulose membrane and is captured by immobilized anti-pLDH antibodies at the test line, producing a visible pink-purple band that confirms malaria infection. The see-through device design allows direct visualization of sample migration and result development, which helps identify invalid tests caused by insufficient sample volume or improper application. This transparency reduces the ambiguity that plagues some lateral flow devices where internal workings are hidden. Sensitivity and specificity have been validated through WHO malaria RDT evaluation programs using panels of wild and cultured parasites. The test detects parasitemia levels above 100 parasites per microliter of blood for both P. falciparum and P. vivax, which is clinically relevant for symptomatic infections requiring treatment. Specificity exceeds 99% when tested against cross-reactive conditions including dengue, leptospirosis, typhoid, and other febrile illnesses common in malaria-endemic areas. Shelf life is 24 to 30 months when stored at 4-30°C, which is critical for stockpiling in tropical climates where cold chain infrastructure is unreliable. The extended temperature stability means the test remains functional even when stored at ambient temperatures in resource-limited settings. Each kit contains individually sealed test devices, buffer vials, blood collection pipettes, and instructions for use. The test requires no special training beyond basic clinical skills and can be performed by nurses, paramedics, or trained community health workers. For distributors supplying national malaria control programs, public health departments, NGO field operations, and private diagnostic laboratories, Advantage PAN Malaria Card represents a WHO-evaluated rapid diagnostic platform with predictable consumption tied to malaria case loads. Sara Wellness has been exporting rapid diagnostic test kits and laboratory reagents from India for 15 years.
Human Serum Coombs Antisera (Antihuman Globulin Reagent)
Blood banking operates on a fundamental requirement that most people never think about until something goes wrong. Every unit of blood transfused must be confirmed compatible with the recipient's immune system. Every pregnant woman screened for antibodies that could harm her unborn child. Every suspected case of hemolytic anemia investigated for antibodies attacking the patient's own red blood cells. None of this happens without Coombs antisera making the invisible antibodies visible. Human Serum Coombs Antisera is the reagent that makes antiglobulin testing possible in blood banks and immunohematology laboratories worldwide. This is antihuman globulin (AHG) reagent used in both direct and indirect antiglobulin tests (Coombs tests) to detect antibodies and complement components bound to red blood cell surfaces or present free in serum. The reagent is produced by immunizing animals (typically rabbits) with human immunoglobulins, which induces production of polyclonal antibodies specific for human IgG antibodies and complement factor C3d. When added to washed red blood cells coated with IgG or complement, the antihuman antibodies bind to the human antibodies and form bridges between adjacent sensitized cells, causing visible agglutination. The direct antiglobulin test (DAT) detects antibodies or complement already bound to red blood cell surfaces in vivo. This test is critical for diagnosing autoimmune hemolytic anemia, investigating hemolytic transfusion reactions, and diagnosing hemolytic disease of the fetus and newborn. The indirect antiglobulin test (IAT) detects free antibodies circulating in serum or plasma. This test is essential for pre-transfusion antibody screening, crossmatching blood units for compatibility, and prenatal antibody screening in pregnant women. Polyspecific Coombs antisera (like the green-colored reagent shown) contains antibodies against both IgG and C3d complement, providing broad-spectrum detection. When the polyspecific reagent produces a positive result, monospecific antisera (anti-IgG alone or anti-C3d alone) are used for follow-up testing to characterize whether red cells are coated with IgG antibodies, complement, or both. This differentiation is clinically important because it helps determine the cause and clinical significance of the positive test. The reagent is typically dyed green using patent blue and tartrazine to allow easy visual identification during laboratory workflows where multiple reagents are used simultaneously. Storage at 2-8°C maintains reagent potency until the expiration date printed on the bottle, typically 18 to 24 months from manufacture. The reagent contains sodium azide (0.1% w/v) as a preservative, which inhibits bacterial growth but requires careful handling and disposal. Each dropper bottle delivers approximately 40 microliters per drop, allowing precise volumetric dosing during testing. The reagent must not be diluted and should not be used if turbid, as turbidity indicates bacterial contamination or protein aggregation that will compromise test performance. For distributors supplying blood banks, hospital transfusion services, reference immunohematology laboratories, and donor screening centers, Coombs antisera represents an essential reagent with consumption directly tied to transfusion volume and prenatal screening programs. Sara Wellness has been exporting immunohematology reagents and blood banking supplies from India for 15 years.
Acon Urine HCG Pregnancy Test Kit
Two lines or one. That's all a pregnancy test comes down to. But ask any pharmacist, clinic manager, or hospital procurement officer how much rides on those two little lines being reliably accurate — and the conversation gets more serious very quickly. Because a false result, in either direction, is not just a product failure. It's a human failure. The ACON urine hCG Pregnancy Test Kit is built on the understanding that reliability is non-negotiable. ACON Laboratories, headquartered in San Diego and one of the world's leading manufacturers of rapid diagnostic tests, uses a chromatographic immunoassay format that detects human chorionic gonadotropin — the pregnancy hormone — in urine at a sensitivity threshold of 25 mIU/mL. That's the level at which hCG is consistently detectable in clinically confirmed early pregnancies. The test is calibrated against the WHO International Standard (3rd edition) and has FDA clearance, two regulatory benchmarks that procurement managers across almost every market in the world recognise and accept as evidence of validated performance. The test works in three minutes. First morning urine is preferred for maximum accuracy, but the test can be performed at any point during the day. Each strip is individually foil-sealed, maintaining integrity until the moment of use. The result reads simply: two lines positive, one line negative, no line in the control window means the test is invalid and should be repeated. Nothing about the procedure requires training or lab equipment — which is precisely why this product moves so effectively across pharmacy chains, government health outreach programmes, antenatal clinics, and over-the-counter retail. For importers and distributors sourcing diagnostic rapid tests in volume, the ACON hCG strip kit offers the rare combination of brand recognition, regulatory compliance, and competitive unit economics. The 50-strip box format is the standard across institutional procurement — hospitals, clinics, and NGO supply chains all order in this configuration. Shelf life runs to 36 months from manufacture under standard storage between 2 and 30°C, which makes inventory planning straightforward even for markets with longer supply chains. Sara Wellness has been exporting diagnostic and lab supply products to buyers across Africa, the Middle East, and Southeast Asia for 15 years. Rapid test kits like the ACON hCG strip are among the highest-velocity SKUs in our catalogue. We understand volume pricing, documentation requirements for IVD products in different export markets, and the importance of consistent batch quality across repeated shipments. Reach out with your volume requirement and we will put together a quote worth looking at.