Albumin Reagent Kit (BCG Method)
Lab Supplies

Albumin Reagent Kit (BCG Method)

Pack Sizes Available

2 x 50 ml (standard kit — 2 x 50ml Albumin Reagent + 1 Albumin Standard Vial)

Product Description

Albumin is one of those analytes that tells a surprisingly large story from a very small sample. It is the most abundant protein in human plasma, making up roughly 60% of total serum protein, and its concentration reflects things as varied as liver function, nutritional status, kidney disease, and hydration. Low albumin can mean liver failure, malabsorption, or nephrotic syndrome. Elevated albumin can flag dehydration. It's one of the most frequently ordered biochemistry tests globally — which means the reagent that runs it needs to be reliable, reproducible, and available consistently. The Albumin Reagent Kit from Microsidd uses the Bromocresol Green (BCG) method, specifically the Doumas et al endpoint colorimetric approach. In plain terms: albumin in the sample binds to the BCG dye under pH conditions of 4.0 to 4.2, producing an albumin-BCG complex with a characteristic colour shift. The intensity of that colour, measured by the analyser at an absorbance peak of 625nm, is directly proportional to the albumin concentration in the sample. The kit is ready-to-use — no preparation steps, no mixing required before the assay. Results are generated in 3 minutes. The detection range runs from 0.130 g/dL to 7.5 g/dL, which covers everything from critically low albumin in acute liver failure to the upper limits of clinically relevant serum levels. The kit arrives as 2 x 50ml of liquid stable albumin reagent plus 1 albumin standard vial — everything needed to run and calibrate the assay. Reagents and the standard are both specially stabilized to ensure consistency across the kit's shelf life. The kit is compatible with semi-automated bench-top analysers and fully automated biochemistry analysers, making it usable across clinic labs, hospital biochemistry departments, and high-throughput reference laboratory settings. For laboratory reagent importers, medical distribution companies, and diagnostic supply chains across the Middle East, Africa, and Southeast Asia — albumin is a bread-and-butter biochemistry test. It is ordered alongside liver function panels, renal function panels, and nutritional assessments every day in every accredited laboratory. A reliable source for this reagent, priced competitively and backed by consistent supply, is worth more to a procurement manager than the occasional discount from an unreliable one. Sara Wellness exports laboratory reagents and biochemistry kits to buyers across multiple international markets. We understand cold-chain logistics, we understand CDSCO registration documentation, and we understand that laboratory procurement managers track expiry dates closely when evaluating suppliers. Every order we handle is accompanied by complete export documentation, and every cold-chain shipment leaves properly packaged for the journey. Fifteen years of this has taught us that the details matter more than the pitch. MOQs are flexible. Combined orders across multiple biochemistry reagent parameters move well and price well together.

Technical Specifications

  • Brand / Manufacturer: Microsidd India — CDSCO registered IVD manufacturer; ML No. MFG/IVD/2018/000004; biochemistry reagent range for clinical laboratory use
  • Method and Principle: BCG (Bromocresol Green) endpoint colorimetric method — Doumas et al; albumin binds BCG dye at pH 4.0–4.2 to form coloured complex; absorbance measured at 625 nm; colour intensity proportional to albumin concentration
  • Detection Range and Result Time: Detection range: 0.130 g/dL to 7.5 g/dL; result time: 3 minutes; endpoint reaction (fixed-time absorbance reading)
  • Kit Contents and Format: 2 x 50ml liquid stable albumin reagent (ready-to-use, no preparation required) + 1 x albumin standard vial; also available in 1 x 100ml and 1 x 250ml single-reagent pack sizes
  • Analyser Compatibility: Compatible with semi-automated and fully automated biochemistry analysers; suitable for cuvette and tube-based systems; multi-reagent calibration compatible; applicable to high-throughput reference laboratory settings
  • Storage and Shelf Life: Store at 2–8°C; do not freeze; protect from direct light; shelf life 18–24 months from manufacture date; sample type: non-haemolysed human serum or plasma; for in vitro diagnostic use only
FAQ

Frequently asked questions

The BCG (Bromocresol Green) method for albumin estimation is a colorimetric assay in which albumin in the serum or plasma sample binds with the BCG dye under acidic conditions (pH 4.0–4.2) to form a blue-green coloured complex. The colour intensity of this complex, measured spectrophotometrically at an absorbance peak of 625nm, is directly proportional to the albumin concentration in the sample. The BCG method, specifically the endpoint colorimetric approach described by Doumas et al, is the most widely adopted method for serum albumin quantification in clinical laboratories globally due to its speed, simplicity, cost-effectiveness, and compatibility with automated biochemistry analysers.

Each Albumin Reagent Kit (2x50ml) contains two bottles of 50ml liquid stable albumin reagent and one vial of albumin standard. The reagent is ready to use — no reconstitution or mixing is required before the assay. The standard is used to calibrate the analyser before running patient samples. For the assay, a small volume of patient serum or plasma is mixed with the albumin reagent, incubated briefly, and the absorbance is measured at 625nm using a semi-automated or fully automated biochemistry analyser. The result is calculated automatically by the analyser against the calibration curve.

The kit is intended for use with human serum and plasma. Non-haemolysed serum is preferred; samples should be collected without prolonged venous stasis. Haemolysis (breakdown of red blood cells causing red discolouration of the serum) can interfere with the BCG colorimetric reaction and should be avoided. Severely lipaemic or icteric samples may also affect absorbance readings. Standard laboratory sample handling practices — prompt centrifugation, visual inspection for haemolysis, and appropriate storage — are recommended before running the assay.

The Albumin Reagent Kit has a confirmed detection range of 0.130 g/dL to 7.5 g/dL, covering the full spectrum of clinically relevant serum albumin concentrations. Low albumin (hypoalbuminaemia) is associated with severe liver disease (hepatic failure, cirrhosis), malabsorption syndromes, protein-losing enteropathy, diarrhoea, eclampsia, and nephrotic syndrome (protein loss through damaged kidneys). Elevated albumin (hyperalbuminaemia) is associated with dehydration. Albumin estimation is routinely ordered as part of liver function tests (LFTs), renal function panels, and nutritional assessment protocols.

The albumin reagent kit is compatible with both semi-automated bench-top biochemistry analysers (such as those commonly found in smaller hospitals, clinics, and reference labs in emerging markets) and fully automated high-throughput biochemistry analysers. Calibration is performed using the albumin standard vial included in the kit. For high-volume automated instruments, calibration frequency should follow the instrument manufacturer's guidelines, with recalibration required if reagent batch changes or if quality control samples fall outside assigned ranges.

The Albumin Reagent Kit must be stored at 2–8°C (refrigerated, not frozen) and protected from direct light. The box label confirms storage at 2–8°C as mandatory throughout the product's lifecycle. Shelf life from manufacture is confirmed at 18–24 months under correct storage conditions, as indicated by the manufacturing and expiry dates printed on the product box. For importers managing cold-chain supply logistics, Sara Wellness ships all reagent kits in temperature-controlled export packaging and can provide batch-specific expiry documentation to assist inventory planning.

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