Erba Uric Acid Biochemistry Reagent Kit (Uricase Method — Liquid Stable)
Lab Supplies

Erba Uric Acid Biochemistry Reagent Kit (Uricase Method — Liquid Stable)

Pack Sizes Available

2 x 50ml single reagent + 1 x 5ml standard

Product Description

Gout has been called the disease of kings and the king of diseases. Both descriptions are accurate in their own way. For centuries it was associated with rich food and rich men — a reputation it earned honestly enough, given that uric acid accumulates when purines are metabolised and purines are plentiful in meat, shellfish, and alcohol. What has changed in recent decades is the clinical picture. Gout is no longer a condition of banquets. It is a condition of the modern metabolic environment — increasingly common, increasingly linked to hypertension, chronic kidney disease, and cardiovascular risk, and increasingly in front of general practitioners, nephrologists, and rheumatologists who need a reliable, consistent uric acid result to make treatment decisions. The Erba Mannheim Uric Acid Biochemistry Reagent Kit is a single-reagent, liquid stable enzymatic assay for the quantitative in vitro determination of uric acid in human serum, plasma, and urine. Erba Diagnostics Mannheim is a German in vitro diagnostics brand with a significant installed base across European and emerging market clinical laboratories, particularly through the Erba Chem semi-automated analyser range that dominates mid-tier hospital laboratory settings across Africa, the Middle East, and South Asia. The chemistry is the Uricase-Trinder / PAP endpoint method. Uricase converts uric acid to allantoin and hydrogen peroxide. The hydrogen peroxide then reacts with 4-aminoantipyrine and DHBS in the presence of peroxidase, producing a red quinoneimine dye. Absorbance of the coloured product is measured at 510nm and is directly proportional to the uric acid concentration. The reaction is complete, specific, and well-characterised — the same chemistry used by most major clinical chemistry platforms globally for serum uric acid quantification. Both pack sizes — 2x50ml (FBCEM0068) and 4x50ml (FBCEM0069) — arrive as a single ready-to-use liquid reagent with no mixing or reconstitution required. On-board stability is a minimum of 30 days at 2–10°C once loaded on the analyser, which is commercially relevant for high-throughput automated analysers running continuous workflows. Linearity extends to 25 mg/dL, covering both normal and severely elevated values including those seen in acute gout flares and tophaceous gout. Known interferents — haemoglobin up to 10 g/L, bilirubin up to 40 mg/dL, and triglycerides up to 2000 mg/dL — do not affect the assay at these concentrations. For laboratory reagent importers and clinical chemistry distributors supplying markets where Erba Chem analysers are widely installed, the Erba reagent line is the natural sourcing choice. Running the instrument's own brand reagents on the Erba Chem platform avoids the compatibility validation burden and is often the procurement default for hospital lab managers who have standardised on this analyser. Sara Wellness exports Erba Mannheim reagents and other Indian-origin and imported clinical chemistry products to wholesale buyers across Africa, the Middle East, and Southeast Asia. We know cold-chain packaging, we know what export documentation your regulatory authority requires, and we have been doing this long enough that the process runs without surprises. Reach out with your volume requirement and we will respond with a real number.

Technical Specifications

  • Brand / Manufacturer: Erba Diagnostics Mannheim GmbH, Germany — global IVD manufacturer; FBCEM0068 (2x50ml) and FBCEM0069 (4x50ml); CE marked; compatible with Erba Chem semi-automated and fully automated analyser series
  • Method and Principle: Uricase-Trinder endpoint colorimetric method (PAP/peroxidase coupled); uricase converts uric acid to allantoin + H₂O₂; H₂O₂ + 4-aminoantipyrine + DHBS + peroxidase → red quinoneimine dye; absorbance measured at 510nm; result proportional to uric acid concentration
  • Reagent Format and Stability: Single reagent (SR), liquid stable, ready to use — no reconstitution or mixing required; on-board stability: minimum 30 days at 2–10°C when refrigerated and uncontaminated; unopened stability to expiry date on label; do not freeze
  • Linearity and Reference Range: Linear to 25 mg/dL (1487 μmol/L); reference range: males 3.4–7.0 mg/dL; females 2.4–5.7 mg/dL; samples above 25 mg/dL should be diluted with NaCl 9 g/L and recalculated; lower detection limit 0.1 mg/dL
  • Sample Types and Interferences: Serum, heparin plasma, EDTA plasma, 24-hr urine (diluted 1:9 in distilled water); non-interfering: Hb ≤10 g/L, bilirubin ≤40 mg/dL, triglycerides ≤2000 mg/dL; do not use haemolytic samples for serum/plasma; calibrate with XL MULTICAL XSYS0034 (ID-MS standardised)
FAQ

Frequently asked questions

Uric acid is the end product of purine metabolism in humans. Unlike most mammals, humans lack the enzyme uricase (in the physiological metabolic pathway), so uric acid cannot be further broken down and must be excreted through the kidneys and intestine. When serum uric acid levels rise above the solubility threshold (approximately 6.8 mg/dL), monosodium urate crystals can deposit in joints, soft tissues, and kidneys, causing gout, tophus formation, and uric acid nephrolithiasis. Chronically elevated uric acid is also associated with hypertension, chronic kidney disease, cardiovascular disease, and metabolic syndrome. Uric acid measurement is routinely ordered for gout diagnosis, monitoring of urate-lowering therapy, and as part of renal and metabolic function panels.

The Erba Uric Acid Reagent Kit uses the Uricase-Trinder (PAP) endpoint colorimetric method. The reaction proceeds in two steps. First, the enzyme uricase oxidises uric acid in the sample to allantoin and hydrogen peroxide. Second, the hydrogen peroxide generated reacts with 4-aminoantipyrine (4-AAP) and DHBS (3,5-dichloro-2-hydroxybenzene sulfonate) in the presence of the enzyme peroxidase, producing a red-coloured quinoneimine dye through the Trinder reaction. The absorbance of this coloured product is measured at 510nm. The intensity of the colour is directly proportional to the uric acid concentration in the sample, which is calculated against a calibrator value.

The Erba Mannheim Uric Acid SR (Single Reagent) Kit is available in two confirmed pack sizes: FBCEM0068 (2 x 50ml single reagent + 1 x 5ml standard) and FBCEM0069 (4 x 50ml single reagent + 1 x 5ml standard). Both are supplied as liquid-stable ready-to-use single reagents requiring no preparation before use. An additional Liquixx-M format in 5 x 10ml is available for semi-automated manual systems. All formats include the uric acid standard vial for calibration. The 4 x 50ml format is suited to high-throughput automated analysers, while the 2 x 50ml is appropriate for lower-volume semi-automated settings.

The Erba Uric Acid Reagent Kit is linear up to 25 mg/dL (1487 μmol/L) as confirmed from the official Erba Mannheim product catalog. This covers the full range from normal values through the highest concentrations typically seen in acute gout flares and tophaceous gout. The reference range for serum uric acid in adults is approximately 3.4–7.0 mg/dL (202–416 μmol/L) for males and 2.4–5.7 mg/dL (143–339 μmol/L) for females. Values above these ranges, particularly above 7 mg/dL, indicate hyperuricaemia and potential gout risk. Each laboratory is advised to establish its own reference range based on its patient population.

The Erba Uric Acid Reagent Kit accepts serum, heparin plasma, EDTA plasma, and urine. For urine testing, a 24-hour urine specimen is required. To prevent uric acid precipitation in urine, 15ml of 5 mol/L NaOH should be added to the urine collector to maintain pH above 8, and the urine sample should be diluted 1:9 with distilled water before testing (multiply results by 10). From the official Erba Mannheim product documentation, the following substances do not interfere with the assay at the concentrations listed: haemoglobin up to 10 g/L, bilirubin up to 40 mg/dL, and triglycerides up to 2000 mg/dL.

Erba Mannheim recommends calibration using the XL MULTICAL calibrator (Catalog No. XSYS0034), which is standardised to ID-MS (isotope dilution mass spectrometry) — the international reference method for clinical chemistry calibration. For quality control, ERBA NORM (Catalog No. BLT00080) and ERBA PATH (Catalog No. BLT00081) are recommended, providing both a normal-range and a pathological-range control serum for two-level QC monitoring. These materials are available separately and are compatible across the Erba Mannheim biochemistry reagent range, making it practical for laboratories standardising on Erba reagents to use a single calibration and QC system across multiple parameters.

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